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Buchan, A., LeCleir, G., Gulvik, C. & González, J. Master recyclers: features and functions of bacteria associated with phytoplankton blooms. Nature Rev. Microbiol. 12, 686–698 (2014). Bridging from the single cell to the cell population – Epo-induced cellular responses and erythroleukemia Alexandrov, L. B. et al. Signatures of mutational processes in human cancer. Nature 500, 415–421 (2013). Kanehisa, M. & Goto, S. KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Res. 28, 27–30 (2000).

The gas can be natural gas (which is mainly methane) or a liquefied petroleum gas, such as propane, butane, or a mixture. Combustion temperature achieved depends in part on the adiabatic flame temperature of the chosen fuel mixture. Duarte, C. M. et al. Is ocean acidification an open-ocean syndrome? Understanding anthropogenic impacts on seawater pH. Estuar. Coast. 36, 221–236 (2013).

Other burners based on the same principle exist. The most important alternatives to the Bunsen burner are: Cultures were supplemented with cytokine-containing medium on day 4, 7, 9 and 11 by replacing half of the medium per well (final cytokine concentrations per well were 50 IU ml −1 interleukin (IL)-2 (Novartis), 25 ng ml −1 IL-7 (Miltenyi, 130-095-367) and 25 ng ml −1 IL-15 (Miltenyi, 130-095-760)). On day 13–15, cells were transferred into cytokine-free medium and on the following day, peptide-specific expansion of T cells was verified by IFN-γ ELISpot or ICS. IFN-γ ELISpot assays were performed as described previously 20. Briefly, cells were plated at a density of 5 × 10 4 cells per well and restimulated with 10 µg ml −1 mutant H3K27M (p14-40) peptide, 10 µg ml −1 wild-type H3 (p14-40) peptide, left unstimulated as a negative control or exposed to PMA/ionomycin (0.02 µg ml −1 and 1 µg ml −1) as a positive control. The assay was stopped after 44 h and spots were quantified using an ImmunoSpot Analyzer (Cellular Technology). TCRβ deep sequencing

Reitman, Z. J. et al. Profiling the effects of isocitrate dehydrogenase 1 and 2 mutations on the cellular metabolome. Proc. Natl Acad. Sci. USA 108, 3270–3275 (2011).Martínez-Vélez, N. et al. The oncolytic virus Delta-24-RGD elicits an antitumor effect in pediatric glioma and DIPG mouse models. Nat. Commun. 10, 2235 (2019). Liu, I. et al. The landscape of tumor cell states and spatial organization in H3-K27M mutant diffuse midline glioma across age and location. Nat. Genet. 54, 1881–1894 (2022).

Arnosti, C. et al. Dynamics of extracellular enzyme activities in seawater under changed atmospheric p CO 2 : a mesocosm investigation. Aquat. Microb. Ecol. 64, 285–298 (2011). Overbeek, R. et al. The subsystems approach to genome annotation and its use in the project to annotate 1000 genomes. Nucleic Acids Res. 33, 5691–5702 (2005). Vogl, C. (2016). Begone Satan! A soul-stirring account of diabolical possession in Iowa. (C. Kapsner, trans.). In C. Vogel, T. Geiger, & C. Kapsner (Eds.), Mary crushes the serpent and Begone Satan! Gilbert: Caritas Publishing. Altschul, S. F. et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389–3402 (1997). Reardon, D. A. et al. Rindopepimut with bevacizumab for patients with relapsed EGFRvIII-expressing glioblastoma (ReACT): results of a double-blind randomized phase II trial. Clin. Cancer Res. 26, 1586–1594 (2020).Vuong, H. G., Ngo, T. N. M., Le, H. T. & Dunn, I. F. The prognostic significance of HIST1H3B/C and H3F3A K27M mutations in diffuse midline gliomas is influenced by patient age. J. Neurooncol. 158, 405–412 (2022). Kirchman, D., K’nees, E. & Hodson, R. Leucine incorporation and its potential as a measure of protein synthesis by bacteria in natural aquatic systems. Appl. Environ. Microbiol. 49, 599–607 (1985). Genomic DNA from tissue, blood or CSF of patient ID 1 was isolated using the DNeasy Blood and Tissue kit (QIAGEN, 69504). Libraries for TCR β-chain deep sequencing were prepared using the hsTCRB kit V4b (Adaptive Biotechnologies) according to the manufacturer’s protocol and sequenced on an Illumina MiSeq device. Sequencing was performed by the Genomics & Proteomics Core Facility (German Cancer Research Center). Data were processed (demultiplexing, trimming, gene mapping) using the immunoSEQ platform from Adaptive Biotechnologies. Motif analysis was carried out using the XSTREME Tool 29 after removing the recurring CAS sequence from all top ten TCRs from TCRβ deep sequencing. Shuffled input sequences were used as control sequences. Statistical analysis Kolker, S. et al. NMDA receptor activation and respiratory chain complex V inhibition contribute to neurodegeneration in d-2-hydroxyglutaric aciduria. Eur. J. Neurosci. 16, 21–28 (2002).

Spreading Liquid Cultures of Bacteria on Agar-Media Plates" (PDF). chemistry.ucla.edu. Archived (PDF) from the original on 2022-10-09 . Retrieved 4 November 2018. Make sure your small change and notes are safe and easily accessible with our Market Trader Money Bags. Made from a highly durable 12 oz. denim with a heavy duty snap fastener on an adjustable belt for ease of use. Langmead, B. & Salzberg, S. L. Fast gapped-read alignment with Bowtie 2. Nature Methods 9, 357–359 (2012).social media platforms to redesign their tools to prevent them from being employed toward harmful political ends and from favouring conflict over consensus; and Kilian, M. et al. MHC class II-restricted antigen presentation is required to prevent dysfunction of cytotoxic T cells by blood-borne myeloids in brain tumors. Cancer Cell 41, 235–251 (2023).